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Characterization of colloid solutions - diffusion coefficient

Using Diatrack, it is possible to track thousands of colloid particles rapidly and precisely. The demonstration illustrates the process of calculating the diffusion coefficient for each particle in a dense colloidal solution.

Surface science - adatom mobility

Measuring the motion of adatoms over chemically clean surfaces informs on energy landscapes and electronic states. In this demonstration, cobalt atom are seen diffusing over a silver surface. They can gain or loose an electron, which has a dramatic effect on their mobility and is manifested as sudden intensity switches. Contributed by Prof. Harald Brune, EPFL.

Protein trafficking

4D datasets are now routinely produced in biological imaging applications to learn more about the motion of proteins and organelles in relation to cell function. This demonstration illustrates the tracking process on a representative biological data set.

Super-resolution tracking.

Super-resolution tracking is the ability to measure position and motion with accuracy better than would be expected, either from consideration of pixel sizes or from the diffraction limit. Diatrack can fit Gaussian intensity profiles over each spot to find their position with sub-pixel precision. In some applications, it is possible to reach a precision better than 1/100 of a pixel. In this demonstration we illustrate the methodology on a simulated data of spots moving on sine tracks.

Manual tracking of fluorescent speckles

Manual tracking is sometimes useful, for example in order to establish a particular tracking methodology. In this demonstration, we illustrate the manual tracking process for fluorescent speckles in the lamellipodium of migrating cells.

Resolving motion correspondence for densely moving objects

Tracking a few well defined objects distant from each other is easy. Unfortunately, this situation occurs rarely in applications. For example, in biological imaging or in granular flows, scenes tend to be crowded with significant changes occurring between images. In this demonstration, we show how Diatrack deals with a difficult sequence borrowed from the literature, using only pairs of successive images and no a priori information. Contributed by Veenman et al. PAMI, vol. 23, No. 1, Jan. 2001.

Tracking demagnetisation grids for cardiology under Magnetic Resonance Imaging (MRI)

Using complex pulse sequences in magnetic resonance imaging, physicians at the Imperial College London are able to generate transient demagnetization grids thus providing an opportunity for characterizing heart motions in detail via object tracking, as shown in the demonstration. Flow movies in particular are used to reveal the average local direction and speed of motion everywhere in the organ over time. Contributed by Dr Protti A., Dr Arridge M., Dr. Gsell W., Biological Imaging Centre, Clinical Sciences Centre, MRC.

Tracking adenovirus particles using fluorescence microscopy

Diatrack allows to quantify how viruses move into and out of cells. The demonstration shows how to set image analysis parameters to track virus particles over a few image frames. Contributed by Prof. Urs Greber, Uni Zuerich.